Research Interests (研究概要)
One of the greatest mysteries surrounding the origin of life is how it came to utilize functional polymers. Understanding how amino acid- and nucleic acid-based macromolecules, such as proteins and RNA, emerged and were maintained through chemical evolution in a non-equilibrium system—along with their functionality, evolvability, and heredity—is crucial. Currently, we employ synthetic biology and analytical chemistry techniques to reconstruct and analyze the properties of primitive proteins, RNA, and their precursors through in vitro evolution experiments. Additionally, we integrate cell-free protein synthesis with enzyme cascades to generate metal cofactors and synthesize various amino acids in a mixotrophic manner within a test tube. This approach serves as a critical step toward bridging the gap between the central dogma and metabolism, advancing the development of self-sustaining artificial cells.
We also use computational methods, such as molecular dynamics (MD) simulations, to explore the precise interactions between fatty acid membranes and small molecules. Furthermore, in collaboration with geochemists, we experimentally investigate how different terrestrial and extraterrestrial environments may facilitate the synthesis of key building blocks and functional biopolymers. Our long-term goal is to construct a universal and plausible scenario for the emergence of recursive, life-like systems. Beyond fundamental research, we believe that the foundational technologies arising from these studies have the potential to drive numerous innovations that will enrich future society. Examples include engineering microorganisms with artificial carbon fixation pathways, discovering and optimizing novel high-performance enzymes, and identifying new peptide sequences for antibody therapeutics. Moving forward, we remain dedicated to unraveling the origins and evolution of life while pioneering breakthroughs that shape the future of humanity.
生命の起源に関する謎の一つに、生命がいかにして機能性ポリマーを獲得し、それを利用するようになったのかという問いがあります。特に、アミノ酸と核酸を基盤とするタンパク質とRNAが、非平衡なエネルギー環境の中でどのように化学進化を遂げ、形成・維持されるに至ったのかを解明することは、生命の機能、進化、遺伝性の理解において極めて重要です。現在、私たちは合成生物学と進化分子工学の手法を駆使し、原始的なタンパク質やRNA、その前駆体の特性を再構築・分析しています。また、無細胞翻訳系と酵素反応を組み合わせることで、試験管内で鉄硫黄クラスターや多様なアミノ酸を、単純な無機物と有機物から合成することに成功しました。このアプローチは、セントラルドグマと代謝を接続した人工細胞の構築に向けた重要な一歩となります。
さらに、分子動力学（MD）シミュレーションを活用し、脂肪酸膜系とアミノ酸・核酸・ペプチドなどの小分子との精密な相互作用やダイナミクスを解析しています。加えて、地球化学者との共同研究により、深海熱水系近傍におけるCO₂流体の貯留環境が、生体関連高分子の合成にどのように寄与するかを実験的に検証しています。これらの研究を通じて、生命のように再帰的に維持されるシステムがどのように出現し得るのか、その最も確からしいシナリオの構築を目指しています。一方、これらの基礎研究から派生する技術は、将来の社会を豊かにする多くのシーズを生み出す可能性を秘めています。例えば、人工的な炭素固定経路を持つ微生物の創出、高活性の新規酵素の探索・改変、さらには抗体医薬を見据えた新規ペプチド配列の設計などがその一例です。私たちは今後も、生命の起源と進化に関する探究を続けるとともに、未来の人間社会への応用を視野に入れた研究を推進していきます。
合成生物学を用いたde novo及び原始的なタンパク質やRNAの合成と機能解析
新規トポロジーを持つタンパク質の探索
深海熱水系近傍のCO2流体と水の二層系における化学進化実験
バイオセンサーを用いた炭素固定酵素の試験管内進化
人工的な炭素固定経路を有する微生物の実験室進化
初期リボソームの構造と機能解析
無細胞翻訳系と共役する鉄硫黄クラスター酵素やアミノ酸合成経路の構築
MDシミュレーションによる脂肪酸膜と低分子の相互作用
非天然アミノ酸を含有する人工/原始ペプチドの配列解析
非リボソーム型ペプチド合成系の起源と進化
機械学習を用いたPET分解酵素の探索及びエンジニアリング
Nano gapシーケンスを用いた1分子レベルでのペプチドや核酸配列の同定
Synthesis and functional analysis of de novo and primitive proteins using synthetic biology
Exploration of proteins with novel topologies
Chemical evolution experiments in a two-phase system of CO2 fluid and water near deep-sea hydrothermal systems
In vitro evolution of CO2 fixation enzymes using protein biosensors
Laboratory evolution of microorganisms with artificial metabolic pathways
Structural and functional analysis of early ribosomes
Construction of iron-sulfur cluster enzymes and amino acid synthesis pathways coupled to a cell-free translation system
Interaction between fatty acid membranes and small molecules through MD simulation
Sequence analysis of artificial/primitive peptides containing non-canonical amino acids
Origin and evolution of non-ribosomal peptide synthesis
ML-driven search and engineering of novel PETases
Using Nano gap sequencing technology to characterize peptide and nucleotide polymers
Wang PH, He MJ, Yi R, Afrin R, Liao KT, Yu WC, Nishikawa S, Sithamparam M, Chen C, Fujishima K, Chandru K, Jia TZ.
Low-Temperature Green Synthesis and Assembly of Poly(Malate-co-Lactate) Gel-Based Microdroplets from Polylactate Plastic Straw Waste
ACS Applied Polymer Materials, pre-print. (2025)
Tagawa S, Hatami R, Morino K, Terazawa S, Akıl C, Johnson-Finn K, Shibuya T, and Fujishima K.
Prebiotic nucleoside phosphorylation in a simulated deep-sea supercritical carbon dioxide-water two-phase environment
Astrobiology, 24(12), 60. 1151-1165. (2024)
Codispoti S, Yamaguchi T, Makarov M, Giacobelli VG, Masek M, Kolar MH, Sanchez Rocha AC, Fujishima K, Zanchetta G, Hlouchova K.
The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability.
Nucleic Acids Research, gkae823. (2024)
Ting WW, Nishikawa S, Yu WC, He MJ, Chen HC, Fujishima K, Sung YJ, Wang KC, Ng IS, Wang PH.
Chemo-enzymatic Synthesis of Coenzyme A Using Copurified Enzymes from Probiotic Escherichia coli Nissle
ACS Sustainable Chemistry & Engineering, 12, 27, 10068–10074. (2024)
Nishikawa S, Yu W, Jia T, He M, Khusnutdinova A, Yakunin AF, Chiang Y, Fujishima K, and Wang P.
Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO2, Ammonium, and α-Ketoglutarate
ACS Catalysis, 14, 7696−7706. (2024)
Furusho A, Ishii C, Akita T, Oyaide M, Mita M, Naraoka H, Takano Y, Dworkin JP, Oba Y, Koga T, Fukushima K, Aoki D, Hashiguchi M, Mita H, Chikaraishi Y, Ohkouchi N, Ogawa NO, Sakai S, Glavin DP, Elsila JE, Parker ET, Aponte JC, McLain HL, Orthous-Daunay FR, Vuitton V, Thissen R, Wolters C, Schmitt-Kopplin P, Ruf A, Isa J, Hertkorn N, Eiler JM, Yoshimura T, Sugahara H, Graham HV, Furukawa Y, Araoka D, Tanaka S, Yoshikawa T, Kabashima F, Sasaki K, Sato H, Yamazaki T, Onose M, Morita M, Kimura Y, Kano K, Aoki J, Fujishima F, Nomura S, Tachibana S, Nakamura T, Noguchi T, Okazaki R, Yabuta H, Yurimoto H, Sakamoto K, Yada T, Nishimura M, Nakato A, Miyazaki A, Yogata K, Abe M, Usui T, Yoshikawa M, Saiki T, Tanaka S, Terui F, Nakazawa S, Okada T, Watanabe S, Tsuda Y and Hamase K.
Enantioselective three-dimensional high-performance liquid chromatographic determination of amino acids in the Hayabusa2 returned samples from the asteroid Ryugu
Journal of Chromatography Open. 100134. (2024)
Nishikawa S, Watanabe H, Terasaka N, Katoh T, Fujishima K.
De Novo Single-Stranded RNA-Binding Peptides Discovered by Codon-Restricted mRNA Display.
Biomacromolecules. 25, 1, 355–365. (2024)
Zeichner SS, Aponte JC, Bhattacharjee S, Dong G, Hofmann AE, Dworkin JP, Glavin DP, Elsila JE, Graham HV, Naraoka H, Takano Y, Tachibana S, Karp AT, Grice K, Holman AI, Freeman KH, Yurimoto H, Nakamura T, Noguchi T, Okazaki R, Yabuta H, Sakamoto K, Yada T, Nishimura M, Nakato A, Miyazaki A, Yogata K, Abe M, Okada T, Usui T, Yoshikawa M, Saiki T, Tanaka S, Terui F, Nakazawa S, Watanabe SI, Tsuda Y, Hamase K, Fukushima K, Aoki D, Hashiguchi M, Mita H, Chikaraishi Y, Ohkouchi N, Ogawa NO, Sakai S, Parker ET, McLain HL, Orthous-Daunay FR, Vuitton V, Wolters C, Schmitt-Kopplin P, Hertkorn N, Thissen R, Ruf A, Isa J, Oba Y, Koga T, Yoshimura T, Araoka D, Sugahara H, Furusho A, Furukawa Y, Aoki J, Kano K, Nomura SM, Sasaki K, Sato H, Yoshikawa T, Tanaka S, Morita M, Onose M, Kabashima F, Fujishima K, Yamazaki T, Kimura Y, Eiler JM.
Polycyclic aromatic hydrocarbons in samples of Ryugu formed in the interstellar medium.
Science. 382(6677):1411-1416. (2023)
Li Y, Kurokawa H, Sekine Y, Kebukawa Y, Nakano Y, Kitadai N, Zhang N, Zang X, Ueno Y, Fujimori G, Nakamura R, Fujishima K, Isa J.
Aqueous breakdown of aspartate and glutamate to n-ω-amino acids on the parent bodies of carbonaceous chondrites and asteroid Ryugu.
Science Advance. 9(50) eadh7845. (2023)
Lin YH, Nishikawa S, Jia TZ, Yeh FI, Khusnutdinova A, Yakunin AF, Fujishima K. and Wang P.
One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass.
Green Chemistry. 25(23), 9896-9907. (2023)
Wang P, Nishikawa S, McGlynn SE, Fujishima K.
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions.
ACS Synthetic Biology. 12, 10, 2887–2896. (2023)
Makarov M, Sanchez Rocha AC, Krystufek R, Cherepashuk I, Dzmitruk V, Charnavets T, Faustino AM, Lebl M, Fujishima K, Fried SD, Hlouchová K.
Early Selection of the Amino Acid Alphabet Was Adaptively Shaped by Biophysical Constraints of Foldability.
Journal of the American Chemical Society. 145 (9), 5320-5329. (2023)
Tretyachenko V, Vymětal J, Neuwirthová T, Vondrášek J, Fujishima K, Hlouchová K.
Modern and prebiotic amino acids support distinct structural profiles in proteins.
Open Biology. (6):220040. (2022)
Jia TZ, Nishikawa S, Fujishima K.
Sequencing the origins of life.
BBA Advances. Volume 2, 100049. (2022)
Fried SD, Fujishima K, Makarov M, Cherepashuk I, Hlouchova K.
Peptides before and during the nucleotide world: an origins story emphasizing cooperation between proteins and nucleic acids.
Journal of the Royal Society Interface. volume19, 187. (2022)
Akıl C, Ali S, Tran LT, Gaillard J, Li W, Hayashida K, Hirose M, Kato T, Oshima A, Fujishima K, Blanchoin L, Narita A, Robinson RC.
Structure and dynamics of Odinarchaeota tubulin and the implications for eukaryotic microtubule evolution.
Science Advances. 8(12):eabm2225. (2022)
Giacobelli VG, Fujishima K, Lepšík M, Tretyachenko V, Kadavá T, Makarov M, Bednárová L, Novák P, Hlouchová K.
In vitro evolution reveals non-cationic protein-RNA interaction mediated by metal ions.
Molecular Biology and Evolution. msac032. (2022)
Reyes SG, Kuruma Y, Fujimi M, Yamazaki M, Eto S, Nishikawa S, Tamaki S, Kobayashi A, Mizuuchi R, Rothschild LJ, Ditzler M, Fujishima K.
PURE mRNA display and cDNA display provide rapid detection of consensus binding motif via high-throughput sequencing.
Biotechnology and Bioengineering, 118(4):1736-1749. (2021)
Tretyachenko V, Voráček V, Souček R, Fujishima K, Hlouchová K.
CoLiDe: Combinatorial Library Design tool for probing protein sequence space.
Bioinformatics, Sep 21:btaa804. (2020)
Jia TZ, 藤島 皓介, 丹羽 達也
液－液相分離と生命の起源
生物工学会誌 第98巻 第5号 228–254（2020）
Wang P, Fujishima K, Berhanu S, Kuruma Y, Jia T, Khusnutdinova AN, Yakunin AF, and McGlynn SE
A bi-functional polyphosphate kinase driving NTP regeneration and reconstituted cell-free protein synthesis.
ACS Synthetic Biology, 9(1), 36-42. (2020)
Takahagi W, Seo K, Shibuya T, Takano Y, Fujishima K, Saitoh M, Shimamura S, Matsui Y, Tomita M and Takai K.
Peptide synthesis under the alkaline hydrothermal conditions on Enceladus.
ACS Earth and Space Chemistry, 3, 11, 2559-2568. (2019)
Urbina J, Patil A, Fujishima K, Paulino-Lima IG, Saltikov C, Rothschild LJ.
A new approach to biomining: Bioengineering surfaces for metal recovery from aqueous solutions.
Scientific Reports, 9, 16422. (2019)
Fujishima, K., Dziomba, S, Yano H, Kebe SI, Guerrouache M, Carbonnier B, and Rothschild, LJ.
The non-destructive separation of diverse astrobiologically relevant organic molecules by customizable capillary zone electrophoresis and monolithic capillary electrochromatography.
International of Jounal of Astrobiology, volume18, issue6, 562-574. (2019)
Vecchioni S, Capece MC, Toomey E, Nguyen L, Ray A, Greenberg A, Fujishima K, Urbina J, Paulino-Lima IG, Pinheiro V, Shih J, Wessel G, Wind SJ, Rothschild LJ.
Construction and characterization of metal ion-containing DNA nanowires for synthetic biology and nanotechnology.
Scientific Reports, 9, 6942. (2019)
Fujishima, K., Wang, K. M., Palmer, J. A., Abe, N., Nakahigashi, K., Endy, D., and Rothschild, L. J.
Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes.
Scientific Reports, 8, 1776. (2018)
Kaneta A, Fujishima K, Morikazu W, Hori H, Hirata A.
The RNA-splicing endonuclease from the euryarchaeaon Methanopyrus kandleri is a heterotetramer with constrained substrate specificity.
Nucleic Acids Reserch, volume46, issue4:1958-1972. (2018)
Froese, T., Campos, J. I., Fujishima, K., Kiga, D., and Virgo, N.
Horizontal transfer of code fragments between protocells can explain the origins of the genetic code without vertical descent.
Scientific Reports, 8, 457. (2018)
Kitadai N., Kameya M. and Fujishima K.
Origin of the Reductive Tricarboxylic Acid (rTCA) Cycle-Type CO2 Fixation: A Perspective.
Life, volume 7, issue 4, 39. (2017)
Matsubara T., Fujishima K, Saltikov CW., Nakamura S. and Rothschild LJ.
Earth analogs for past and future life on Mars: Isolation of perchlorate resistant halophiles from Big Soda Lake.
International of Jounal of Astrobiology, Volume 16,Issue 3.(2016)
Paulino-Lima IG., Fujishima K., Navarrete JU, Galante D, Rodrigues F, Azua-Bustos A. and Rothschild LJ.
Extremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations.
Journal of Photochemistry and Photobiology B: Biology, volume 163:327-36. (2016)
Holm Hansen AC., Paulino-Lima IG., Fujishima K., Rothschild LJ. and Jensen PR.
Draft Genome Sequence of Hymenobacter sp. Strain AT01-02, Isolated from a Surface Soil Sample in the Atacama Desert, Chile.
Genome Announcements, volume4, No.1: e01701-15. (2016)
Fujishima K., Venter C., Wang K., Ferreira R, and Rothschild LJ.
An overhang-based exon shuffling method for creating a customized random DNA library.
Scientific Reports, 5: 9740. (2015)
Ikeda KT., Hirose Y., Hiraoka K, Noro E., Fujishima K., Tomita M. and Kanai A.
Identification, expression, and molecular evolution of microRNAs in the "living fossil" Triops cancriformis (tadpole shrimp).
RNA, 21(2):230-242. (2015)
Hirata A, Fujishima K, Yamagami R, Kawamura T, Banfield JF, Kanai A. and Hori H.
X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity
Nucleic Acids Reserch, Volume 40,Issue 20:10554-66. (2012)
Sugahara J., Fujishima K., Nunoura T., Takaki Y., Takami H., Takai K., Tomita M. and Kanai A.
Genomic Heterogeneity in a Natural Archaeal Population Suggests a Model of tRNA Gene Disruption.
PLoS ONE,7(3):e32504. (2012)
Hamashima K., Fujishima K., Masuda T., Sugahara J., Tomita M. and Kanai A.
Nematode-specific tRNAs that decode an alternative genetic code for leucine.
Nucleic Acids Reserch,Volume 40,Issue 8:3653-62 (2011)
Murakami S., Fujishima K., Tomita M. and Kanai A.
Metatranscriptomic analysis of microbes in an ocean-front deep subsurface hot spring reveals novel small RNAs and type-specific tRNA degradation.
Applied and Environmental Microbiology,Volume 78,No. 4:1015-1022 (2011)
Fujishima K., Sugahara J., Miller CS., Baker BJ., Di Giulio M., Takesue K., Sato A., Tomita M., Banfield JF. and Kanai A.
A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity.
Nucleic Acids Research, Volume 39, Issue 22:9695-704 (2011)
Fujishima K., Sugahara J., Tomita M. and Kanai A.
Large-scale tRNA intron transposition in the archaeal order Thermoproteales represents a novel mechanism of intron gain.
Molecular Biology and Evolution, Volume 27, Issue 10:2233-43 (2010)
Takane K., Fujishima K., Watanabe Y., Sato A., Saito N., Tomita M. and Kanai A.
Computational prediction and experimental validation of evolutionarily conserved microRNA target genes in bilaterian animals.
BMC Genomics, 11,No. 101(2010)
Kitamura S., Fujishima K., Sato A., Tsuchiya D., Tomita M. and Kanai A.
Characterization of RNase HII substrate recognition using RNase HII-Argonaute chimeric enzymes from Pyrococcus furiosus.
Biochemical Journal, 426(3):337-44 (2010)
Fujishima K., Sugahara J., Kikuta K., Hirano R., Sato A., Tomita M. and Kanai A.
Tri-split tRNA is a transfer RNA made from three transcripts that provides insight into the evolution of fragmented tRNAs in archaea.
PNAS, 106(8):2683-7 (2009).
Sugahara J., Fujishima K., Morita K., Tomita M. and Kanai A.
Disrupted tRNA gene diversity and possible evolutionary scenarios.
Journal of Molecular Evolution,volume 6,No. 497 (2009)
Fujishima K., Sugahara J., Tomita M. and Kanai A.c
Sequence Evidence in the Archaeal Genomes that tRNAs Emerged Through the Combination of Ancestral Genes as 5' and 3' tRNA Halves.
PLoS ONE, 25(12):2709-16 (2008)
Sugahara J., Kikuta K., Fujishima K., Yachie N., Tomita M. and Kanai A.
Comprehensive analysis of archaeal tRNA genes reveals rapid increase of tRNA introns in the order thermoproteales.
Molecular Biology and Evolution, Volume 25, Issue 12:2709-16 (2008)
Fujishima K., Komasa M., Kitamura S., Tomita M. and Kanai A.
Comparison and characterization of proteomes in the three domains of life using 2D correlation analysis.
Progress of Theoretical Physics Supplement_, Volume 173:206-18 (2008)
Fujishima K., Komasa M., Kitamura S., Suzuki H., Tomita M. and Kanai A.
Proteome-wide prediction of novel DNA/RNA-binding proteins using amino acid composition and periodicity in the hyperthermophilic archaeon Pyrococcus furiosus.
DNA Research, Volume 14, Issue 3,:91-102 (2007)
Review (レビュー)
Fujishima K and Kanai A. tRNA gene diversity in the three domains of life. Front Genet, (5):142. (2014)
Fujishima K and Kanai A. Split Gene. Brenner’s Encyclopedia of Genetics, 2nd edition, 543-544. (2013)
Books (書籍)
連載：新分野創造「シンセティック・アストロバイオロジー」 細胞工学，学研メディカル秀潤社
第1回「アストロバイオ×合成生物学」 Vol.34 No.2 (2015)
第2回「火星移住と合成生物学（前編）」Vol.34 No.4 (2015)
第3回「火星移住と合成生物学（後編）」Vol.34 No.7 (2015)
第4回「第二の生命と合成生物学（前編）」Vol.34 No.12 (2015)
第5回「第二の生命と合成生物学（後編）」Vol.35 No.1 (2016)
第6回「生命の起源と合成生物学」Vol.35 No.3 (2016)
おもろいバイオロジー第8回 拝啓，未来のアストロバイオロジストへ 細胞工学 Vol.32 No.12 (2013)，学研メディカル秀潤社
Fujishima K, Sugahara J, Kanai A. 三つの遺伝子に断片化したtRNAの発見. 生化学 Vol.82(7):606-12 (2010), 日本生化学会
伊藤 昇平、西川 将太、寺坂 尚紘、藤島 皓介 「PUREシステムを用いたマロニルCoAバイオセンサーの最適化」 第19回無細胞生命科学研究会 (Mar 2025)
[Invited] Fujishima K. "Evolution of Life on Earth - Where do we come from?", JAGFOS2024 (Oct 2024)
[Invited] Fujishima K. "Expanding the cell-free synthetic biology", University of Manchester – Tokyo Institute of Technology Joint Workshop (June 2024)
[Invited] Fujishima K. "Astrobiology x Exoplanetary science: what are the next steps for creating new synergy?", JpGU Meeting 2024 (May 2024)
Tagawa S, Hatami R, Morino K, Terazawa S, Akil C, Johnson-Finn K, Shibuya T, Fujishima K. "The Function of Deep-sea Supercritical CO2-water System in Prebiotic Chemical Evolution", AbSciCon 2024 (May 2024)
Fujishima K. "Expanding the reconstituted
cell-free TX/TL system", Build-a-Cell seminar series (May 2024)
西川 将太、Yu WC、He MJ、Jia TZ、Khusnutdinova A、Yakunin AF、Chiang YR、藤島 皓介、Wang PH「廃棄プラスチックを食べてアミノ酸を自己再生産する PURE システム」、第 18 回無細胞生命科学研究会 (Mar 2024)
藤島皓介「生命の最終共通祖先以前のリボソームの再構成と機能検証」第12回 宇宙における生命ワークショップ(Feb 2024)
Wang PH、西川将太、McGlynn SE、藤島皓介 「酸素除去系と鉄硫黄クラスター生合成マ シナリーを利用した無細胞鉄硫黄タンパク質合成系の確立」、「細胞を創る」研究会 16.0、東京 (日本)2023 年 9 月 25-26 日
Tagawa S, Hatami R, Fujishima K. "Deep-sea supercritical CO2-water environment drives prebiotic nucleoside phosphorylation", Australasia Astrobiology Meeting 2023 (June 2023)
Nishikawa S, Wang PH, McGlynn SE, Fujishima K. "De novo synthesis of iron-sulfur proteins using recombinant SUF with O2-scavenging cascade in a cell-free system", SynCell2023 (May 2023)
Tagawa S, Hatami R, Fujishima K. "Prebiotic phosphorylation under deep-sea supercritical CO2-water two-phase environment", JpGU Meeting 2023 (May 2023).
Tagawa S, Hatami R, Fujishima K. "Prebiotic nucleoside phosphorylation under deep-sea supercritical CO2-water two-phase environment", The 47th SSOEL Annual Meeting (Mar 2023)
Fujishima K. "Abiotic vs Biotic polymerization", 11th ELSI Symposium (Mar 2023)
Fujishima K. "How to make complexed biomolecules", 82nd Fujihara Seminar (Feb 2023)
Nishikawa S., Watanabe H., Terasaka N., Katoh T., Fujishima K. "Discovery of de novo single-strand RNA binding peptides", 分子生物学会第45回年会 (Dec 2022)
[Invited] 藤島皓介「生命の起源に分子進化工学で迫る」第5回ExCELLSシンポジウム (Dec 2022)
Tagawa S. "Prebiotic nucleotide synthesis under deep-sea scCO2/water environment", 3rd IOoL2022, Montreal, CANADA (Aug 2022)
Nishikawa S, Watanabe H, Terasaka N, Katoh , Fujishima K. "Screening de novo RNA-binding peptides using codon-depleted mRNA display", AbSciCon2022, Online (May 2022)
村山華子, 山口智子, 藤島皓介「リボソームの進化から迫る遺伝暗号の起源」ISAS宇宙生命探査シンポジウム(March 2022)
山口智子, 西川将太, 藤島皓介「初期リボソームにペプチジル活性はあるのか」第46回 生命の起原および進化学会学術講演会オンライン (March 2022)
田川翔大朗, 播田實りょう太, 藤島 皓介 「深海の超臨界二酸化炭素環境を模擬したプレバイオティックなヌクレオシドのリン酸化反応」第46回 生命の起原および進化学会学術講演会オンライン (March 2022)
[Invited] 藤島皓介「アストロバイオロジー研究の最前線 〜宇宙に生命はいるのか？〜」日本動物学会関東支部第74回大会 (March 2022)
Fujishima K. Exploring the sequence space of de novo and primordial protein-RNA interaction, CAS-JSPS Bilateral Seminar, Online (August 2021)
[Invited] 藤島皓介「ディスプレイ法で探るRNA-タンパク質相互作用の分子進化 」第44回日本分子生物学会 (Dec 2021)
Fujishima K. PURE mRNA display for exploring RNA-binding protein sequence space, RNA Meeting 2021, Online (July 2021)
西川将太, 渡辺秀教, 寺坂尚紘, 加藤敬行, 藤島皓介「コドンを限定したmRNAディスプレイ法による新規RNA結合ペプチドの探索」 第15回無細胞生命科学研究会, オンライン (March 2021)
Nishikawa S, Watanabe H, Terasaka N, Katoh T, Fujishima K. Screening de novo RNA-binding peptides from a sequence space with limited amino acid diversity, ASTROBIOLOGY AUSTRALASIA MEETING 2020, Online (September 2020)
藤島皓介, Reyes SG, 車兪澈.「無細胞翻訳系を利用したクリー ンなmRNA/cDNAディスプレイ法の確立 」第14回無細胞生命科学研究会 (November 2019)
[Invited] Fujishima K, Kitadai N, Johnson FK and Kasama T, Probing the Peptide Sequence Space on Iron Sulfide Surface. AbSciCon 2019, Bellevue, WA, USA (June 2019)
[Invited] Fujishima K. Peptide oriented origin of life scenario. COSPAR2018 42nd assembly, Pasadena, CA, USA (July 2018)
[Invited] Fujishima K. Protein engineering and in vitro evolution studies for the origins of life. Prague Protein Spring 2018, Prague, Czech Republic (May 2018)
Fujishima K, Greenberg D, Kobayashi A, Kuruma Y, Mizuuchi R, Rothschild LJ and Ditzler MA. In vitro RNA-peptide co-evolution system for screening ATP-binding RNP. XVIIIth International Conference on the Origin of Life, San Diego, CA, USA (July 2017)
Fujishima K, Greenberg D, Kuruma Y, Mizuuchi R, Rothschild LJ and Ditzler MA. Can peptide-RNA coevolution provide unique opportunities for evolutionary innovation? AbSciCon 2017 Mesa, AZ, USA (May 2017)
Fujishima K. A tale of Origin of Life spun by two polymers. Public lecture for 5th ELSI Symposium, Tokyo, Japan (Jan 2017)
[Invited] Fujishima K, Polymerization of Biomolecules Using Deep-Sea Water-Liquid CO2 Reactor, From Minerals to Enzymes. EON Workshop on Electrochemistry at the origin of life , Tokyo, Japan (Nov 2016)
[Invited] Fujishima K, Rothschild LJ, Mizuuchi R, Shibuya T, Kitadai N, Kuruma Y, Ditzler M. Synthetic Astrobiology approach towards origin and evolution of RNA and proteins,「細胞を創る」研究会 9.0, (How to make a Cell 9.0) Tokyo, Japan (Nov 2016)
Fujishima K, Dziomba S, Takahagi W, Shibuya T, Takano Y, Guerrouache M, Carbonnier B, Takai K, Rothschild L and Yano H. A fly-through mission strategy targeting peptide as a signature of chemical evolution and possible life in Enceladus plumes. Conference on Enceladus and the Icy Moons of Saturn. Boulder, Colorado, USA (July 2016)
Fujishima K, Carbonnier B, Guerrouache M, and Rothschild LJ. To the Plumes of Icy Moon and Elsewhere: in situ analysis of extraterrestrial organics and biomarkers using Miniature Electrochromatographic Monolithic Column (MEMoC). AbSciCon 2015 Chicago, IL, USA (June 2015)
Fujishima K, Wang K, Ferreira R, Rothschild L. De novo synthesis and functional study of primitive polypeptides in the prebiotic Protein World. COSPAR 2014, Moscow, Russia (Oct 2014)
Fujishima K, Wang K, Ferreira R, Rothschild L. De novo synthesis and functional study of primitive polypeptides in the prebiotic Protein World. Origins 2014, Nara, Japan (Jun 2014)
[Invited] Fujishima K. 地球生命がゲノムDNAを獲得するまで. 第8回日本ゲノム微生物学会年会, Tokyo, Japan (Mar 2014)(English translation: Path towards DNA for Terrestrial life, 8th Conference of Genome Microbiology)
Fujishima K, Venter CE, Rothschild L. An experimental exploration of a prebiotic peptide world. Gordon Research Conference: Origin of Life Galveston, TX, USA (Jan 2012)
伊藤昇平、寺坂尚宏、西川翔太、藤島皓介 「無細胞翻訳バイオセンサーと液滴ソーティングを用いたCO2固定酵素の指向性進化」第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
Tang I, Nishikawa S, Wang PH, Fujishima K "Biochemical Synthesis of Amino Acids Derived from PLA Plastics for Cell-Free Protein Synthesis" 第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
Tanaka Y, Yagi S, Tagami S, Gondhalekar R, Fujishima K "Capturing metamorphic conformational changes between DPBB/DZBB structure: implication to early protein evolution" 第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
Seya K, Terazawa S, Longo LM and Fujishima K "Searching for Unexplored Diversity Among Highly Conserved Phosphate Binding Loops" 第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
足立希美、増田直旺、寺澤昌平、藤島皓介 「ランダムRNAの連結と分解のサイクルから生じる配列および構造の特徴解析」第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
Abe R, Ginter T, Fujishima K "Analysis of the interactions between fatty acid membranes and nucleic acid monomers using molecular dynamics simulation" 第47回日本分子生物学会 / The 47th Annual Meeting of the Molecular Biology Society of Japan (Nov 2024)
Gondhalekar R, Tagami S, Yagi S, Fujishima K "Exploring a Nucleoprotein Origin of Life through Ribozyme-Assisted In Vitro Evolution of Protein Polymerases" 29th tRNA Conference (Nov 2024)
Tang I, Nishikawa S, Wang PH, Fujishima K "Development of biochemical synthesis of serine, glycine and glutamate from biodegradable plastic in a cell-free system" 「細胞を創る」研究会 17.0 (Nov 2024)
西川 将太、Wen-Chi Yu、Tony Z. Jia、Ming-Jing He、Anna Khusnutdinova、Alexander F. Yakunin、Yin-Ru Chiang、藤島 皓介 、Po-Hsiang Wang「ポリ乳酸プラスチックを基質としたアミノ酸自己再生 PURE システム」「細胞を創る」研究会 17.0 (Nov 2024)
堀野瑞紀、藤島皓介 「長期実験室進化により人工炭素固定経路で生育する微生物の創出」日本微生物生態学会 第37回大会 (Oct 2024)
Gondhalekar R, Tagami S, Yagi S, Fujishima K "Exploring a Nucleoprotein Origin of Life through Ribozyme-Assisted In Vitro Evolution of Protein Polymerases" 25th Annual Meeting of the RNA Society of Japan (June 2024)
Ginter T and Fujishima K. "Plausible Peptide-Protocell Processes: Fatty acid membranes and small molecule interactions" 21st International Union for Pure and Applied Biophysics (IUPAB) (June 2024)
Wang PH, Nishikawa S., McGlynn SE, and Fujishima K. "One-pot cell-free synthesis of [4Fe-4S] proteins using recombinant SUF system under aerobic conditions" Biocatalysis Faraday Discussion (May 2024)
Morino K, Tagawa S, Terazawa S, and Fujishima K. “Quantitative Understanding of Nucleoside Phosphorylation in Supercritical CO2 / Water Two-Phase System” JpGU Meeting 2024 (May 2024)
Tagawa S, Hatami R, Morino K, Terazawa S, and Fujishima K. "The roles of subseafloor supercritical CO2-water two-phase environment in prebiotic chemical evolution", 12th ELSI symposium (Jan 2024)
Nishikawa S., Yu WC, Jia TZ, He MJ, Khusnutdinova A, Yakunin AF, Fujishima K, and Wang PH. "Bottom-up construction of a polylactate-metabolizing chemoheterotrophic system" 12th ELSI symposium (Jan 2024)
Gondhalekar R, Tagami S, Yagi S, Fujishima K. "Deciphering the origin of protein polymerases using an RNA-protein coevolution system" 12th ELSI symposium (Jan 2024)
Morino K, Tagawa S, Terazawa S, Tan S and Fujishima K. “Quantitative Understanding of Nucleoside Phosphorylation in Supercritical CO2/water Two-Phase System” 12th ELSI symposium (Jan 2024)
水島良太、鈴木直也、逸見明央、島田幸輝、神谷幸太郎、藤島皓介「機械学習を用いた酵素機能予測モデルによる新規PETaseの発見」第75回日本生物工学会大会 (Sep 2023)
Nishikawa S, Watanabe H., Terasaka N, Katoh T, and Fujishima K. "Exploring RNA-binding capability within the sequence space of restricted amino acid alphabet" Origins2023 (Aug 2023)
Tagawa S, R. Hatami, K. Morino, S. Terazawa, Fujishima K. “Prebiotic organic phosphorylation in deep-sea supercritical CO2-water environment using hydroxyapatite” Water-Rock Interaction-17, (Aug 2023)
Nishikawa S, Wang PH, McGlynn SE, Fujishima K. "De novo synthesis of iron-sulfur proteins using recombinant SUF system in a cell-free system" SynCell2023 (May 2023)
LIU Y. "High-throughput ancestral protein thermostability analysis towards thermal adaptation of early life on earth" AbGradCon2023 (May 2023)
Gondhalekar R. "Scaling of protein function across the Tree of Life" AbGradCon2023 (May 2023)
Terazawa S., Nishikawa S., Longo L., Fujishima K., "De novo generation of RNA-binding protein from diverse structure library" 11th ELSI symposium (Mar 2023)
Nishikawa S., Wang PH., McGlyn SE., Fujishima K., "One-pot de novo synthesis of [4Fe-4S] proteins using recombinant SUF system under aerobic conditions" 11th ELSI symposium (Mar 2023)
LIU Y, Yokobori S., Fujishima K. "High-throughput protein thermostability analysis towards thermal adaption of early life on earth" 第45回日本分子生物学会 / The 45th Annual Meeting of the Molecular Biology Society of Japan (Dec 2022)
寺澤昌平、西川将太、Longo Liam、藤島皓介「タンパク質の構造ライブラリから新規RNA結合ドメインを同定する」第45回日本分子生物学会 / The 45th Annual Meeting of the Molecular Biology Society of Japan (Dec 2022)
Nishikawa S., Watanabe H., Terasaka N., Kato T., Fujishima K., "Discovery of de novo single-strand RNA binding peptides" HFSP awardees meeting (Aug 2022)
Nishikawa S., Xia Y., Yanase K., Hlouchová K., Fried S.D., Fujishima K., "High-throughput directed evolution of aminoacyl-tRNA synthetases using mRNA display" HFSP awardees meeting (Aug 2022)
田川翔大朗、播田實りょう太、藤島皓介「生命の起源研究における異分野融合」超異分野学会 大阪大会、大阪 (Aug 2022)
LIU Y., Yokobori S., Fujishima K. "High-throughput protein thermostability analysis towards thermal adaption of early life on earth" 第22回日本蛋白質科学会 年会第2回蛋白質科学会若手の会 研究交流会 (June 2022)
寺澤昌平、西川将太、Longo Liam、藤島皓介「二次構造シャッフリング法でタンパク質の未開拓構造を探索する」第22回日本蛋白質科学会 年会第2回蛋白質科学会若手の会研究交流会 (June 2022)
Tagawa S, Hatami S, Fujishima K. "Prebiotic nucleoside phosphorylation under simulated deep-sea supercritical carbon dioxide environment" JpGU Joint Meeting 2022, Online (June 2022)
西川将太、渡辺秀教、寺坂尚紘、加藤敬行、藤島皓介「アミノ酸種を限定したmRNAディスプレイ法による新規RNA結合ペプチドの探索」第44回日本分子生物学会 (Dec 2021)
柳瀬航太、西川将太、藤島皓介「原始アミノアシルtRNA合成酵素を再構成しタンパク質構成アミノ酸の変遷に迫る」第44回日本分子生物学会 (Dec 2021)
寺澤昌平、西川将太、藤島皓介「二次構造シャッフリングによって合成したde novoタンパク質のRNA結合能によるスクリーニング」第44回日本分子生物学会 (Dec 2021)
LIU Y, Yokobori S, Fujishima K. "Exploring the evolutionary thermostability of ancestral proteins by combining ancestral sequence reconstruction and mRNA display methods" 第44回日本分子生物学会 (Dec 2021)
西川将太、渡辺秀教、寺坂尚紘、加藤敬行、藤島皓介「mRNAディスプレイ法による新規ポリ(C)RNA結合ペプチドの探索」 「細胞を創る」研究会13.0, オンライン (November 2020)
Nishikawa S., Watanabe H., Terasaka N., Katoh T., Fujishima K., “Screening RNA-binding peptides using restricted set of amino acids” JpGU-AGU Joint Meeting 2020, Online (July 2020)
Nishikawa S., Watanabe H., Terasaka N., Katoh T., Fujishima K., “Screening RNA-binding peptides using restricted set of amino acids; Random peptide library selection revealed a tripeptide motif with the affinity to PolyC mRNA” ELSI 8th International Symposium, Tokyo, Japan (February 2020)
西川将太, 渡辺秀教, 寺坂尚紘, 加藤敬行, 藤島皓介「アミノ酸種を限定したペプチドライブラリによるPoly-(ACGU)RNA結合アプタマーの新規探索」第14回無細胞生命科学研究会, 神奈川 (November 2019)
西川将太, 渡辺秀教, 寺坂尚紘, 加藤敬行, 藤島皓介「アミノ酸種を限定したペプチドライブラリによるPoly-(ACGU)RNA結合アプタマーの新規探索」「細胞を創る」研究会12.0, 愛媛 (October 2019)
藤島皓介, 車兪澈, 水内良, Lynn Rothschild, Mark Ditzler 「試験管内翻訳系を利用したRNAとペプチドの共進化実験」日本進化学会第20回大会 (August 2018)